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  • Title: Incorporation of 14C-amino acids into rat hypothalamus in vitro.
    Author: Kordić D, Kniewald Z.
    Journal: Endocrinol Exp; 1977 Mar; 11(1):3-10. PubMed ID: 322995.
    Abstract:
    Biosynthesis of peptides in vitro in male rat hypothalamic fragments wasinvestigated by incubating the tissue with 14C-labelled leucine, proline and glycine. Depending on the amino acid used, 4 or 6 labelled peptides were extracted from the tissue fragments with organic solvent mixture. The incorporation of the label into peptides was time dependent. LH-RH and oxytocin thus synthesized were identified by thin layer chromatography and high voltage electrophoresis using cold LH-RH and oxytocin as standards.
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