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  • Title: Simple and Rapid Detection of Glycoforms by "Lectin Inhibition" Assay.
    Author: Hoshi K, Hashimoto Y, Ito H.
    Journal: Methods Mol Biol; 2020; 2132():165-171. PubMed ID: 32306325.
    Abstract:
    Glycoforms are otherwise identical proteins with different glycosylation. A lectin, Sambucus sieboldiana agglutinin (SSA), specifically binds glycoforms having α2,6-sialyl residues. The binding is found to inhibit antigen-antibody reaction; e.g., SSA inhibits anti-transferrin antibody binding to α2,6-sialylated transferrin (Tf) (SSA inhibition). SSA inhibition is not observed with other Tf glycoforms, indicating that the inhibition is glycoform-specific. Here we describe the application of SSA inhibition to ELISA as a specific assay for quantifying α2,6-sialylated Tf.
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