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Title: Isolation and characterization of Hena1 - a novel Erwinia amylovora bacteriophage. Author: Besarab NV, Akhremchuk AE, Zlatohurska MA, Romaniuk LV, Valentovich LN, Tovkach FI, Lagonenko AL, Evtushenkov AN. Journal: FEMS Microbiol Lett; 2020 May 01; 367(9):. PubMed ID: 32319510. Abstract: Fire blight, caused by plant pathogenic bacterium Erwinia amylovora, is one of the most important diseases of Rosaceae plants. Due to the lack of effective control measures, fire blight infections pose a recurrent threat on agricultural production worldwide. Recently, bacterial viruses, or bacteriophages, have been proposed as environmentally friendly natural antimicrobial agents for fire blight control. Here, we isolated a novel bacteriophage Hena1 with activity against E. amylovora. Further analysis revealed that Hena1 is a narrow-host-range lytic phage belonging to Myoviridae family. Its genome consists of a linear 148,842 bp dsDNA (48.42% GC content) encoding 240 ORFs and 23 tRNA genes. Based on virion structure and genomic composition, Hena1 was classified as a new species of bacteriophage subfamily Vequintavirinae. The comprehensive analysis of Hena1 genome may provide further insights into evolution of bacteriophages infecting plant pathogenic bacteria.[Abstract] [Full Text] [Related] [New Search]