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  • Title: Circular dichroism of isolated ricin A- and B-chains.
    Author: Wawrzynczak EJ, Drake AF, Thorpe PE.
    Journal: Biophys Chem; 1988 Sep; 31(3):301-5. PubMed ID: 3233304.
    Abstract:
    An analysis of the circular dichroism (CD) spectra of isolated ricin A- and B-chains revealed several bands not apparent in the spectrum of intact ricin. Arithmetic combination of the A- and B-chain spectra gave a composite spectrum resembling that of native ricin, indicating that the two chains did not undergo any major conformational change upon dissociation. The addition of lactose to the B-chain at pH 7.2 caused a slight perturbation of a tryptophan-derived negative CD band centred at 283 nm without change to the overall structure of the polypeptide.
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