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  • Title: Ultrasound Assisted Cascade Extraction of Oil, Vitamin E, and Saccharides from Roselle (Hibiscus Sabdariffa L.) Seeds.
    Author: Le BA, Okitsu K, Imamura K, Takenaka N, Maeda Y.
    Journal: Anal Sci; 2020 Sep 10; 36(9):1091-1097. PubMed ID: 32336730.
    Abstract:
    Roselle seeds, a waste biomass of the roselle calyx processing industry, were utilized to recover valuable compounds of oil, vitamin E, and water-soluble saccharides. Firstly, ultrasound-assisted extraction (UAE) and conventional stirring extraction were conducted for saccharide extraction, and the advantage of UAE was confirmed. Secondly, oil, vitamin E, and saccharides extracted from Vietnamese roselle seeds by UAE were analyzed for the first time. Oil of tri-, di-, and mono-glycerides, fatty acids of linoleic-, oleic-, palmitic-, and stearic-acids, vitamin E of γ- and α-tocopherol, and saccharides of sucrose, raffinose, stachyose, etc. were identified, and the amounts of these components were compared with those in other country's roselle seeds. Thirdly, cascade extraction of oil, vitamin E, and saccharides by UAE was investigated with solvents of hexane, hexane:ethyl acetate binary solvent, and water. The results indicated that the order of using solvents was very important for high and selective extraction: the best order to recover oil (almost 100%), vitamin E (95.7%), and saccharides (86.2%) was hexane, and then water.
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