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  • Title: Horizontal two-dimensional electrophoresis with immobilized pH gradients using PhastSystem.
    Author: Görg A, Postel W, Günther S, Friedrich C.
    Journal: Electrophoresis; 1988 Jan; 9(1):57-9. PubMed ID: 3234335.
    Abstract:
    Protocols for horizontal two-dimensional electrophoresis with immobilized pH gradients in the first dimension were modified for horizontal micro two-dimensional electrophoresis using PhastSystem. Different equilibration conditions of the first-dimensional immobilized pH gradient gel strip prior to second-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis were evaluated. Silver stained two-dimensional patterns were obtained within 3.5 h.
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