These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: High-performance liquid chromatographic separation of peptides on a diol-Gly-Phe-Phe tripeptide-bonded phase.
    Author: Pinkerton TC, Koeplinger KA.
    Journal: J Chromatogr; 1988 Dec 23; 458():129-45. PubMed ID: 3235630.
    Abstract:
    The retention characteristics of some selected peptides (mol. wt. less than 2000 a.m.u.) have been investigated on a diol-Gly-Phe-Phe partitioning phase, bound to 5-microns porous silica. The hydrophobic, positively charged peptides can be separated with mild mobile phases, containing only acetonitrile and phosphate buffer. The peptide selectivity of the diol-Gly-Phe-Phe-bonded phase is uniquely different from that of a C8 column. The dependence of capacity factors on mobile phase pH, ionic strength, and organic solvent concentration demonstrated that the partitioning mechanisms of the diol-Gly-Phe-Phe phase involve multifunctional reversed-phase and cation-exchange processes.
    [Abstract] [Full Text] [Related] [New Search]