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  • Title: Detecting β-Galactosidase-Labeled Cells.
    Author: Rodig SJ.
    Journal: Cold Spring Harb Protoc; 2020 May 01; 2020(5):099739. PubMed ID: 32358051.
    Abstract:
    β-Galactosidase has been used extensively both as a label in enzyme immunoassays and for immunocytochemistry. One good substrate is 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside (X-gal), which gives an intense blue product. The product is stable and insoluble in alcohol as well as H2O.
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