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  • Title: Analysis of Serum Anti-Zika Virus Antibodies by Focus Reduction Neutralization Test.
    Author: Smith JL, Hirsch AJ.
    Journal: Methods Mol Biol; 2020; 2142():73-80. PubMed ID: 32367359.
    Abstract:
    Zika virus (ZIKV) is a newly emerged mosquito-borne flavivirus that has been associated with birth defects of babies born to ZIKV-infected mothers. Neutralization activity of serum derived from ZIKV infected and vaccinated individuals is a critical component for characterizing immune response to infection and vaccine efficacy. This protocol describes a modified plaque reduction neutralization 50 (PRNT50) assay that includes an immunostaining step to improve reproducibility and throughput of the assay.
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