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  • Title: 5S rRNA genes in Pisum: sequence, long range and chromosomal organization.
    Author: Ellis TH, Lee D, Thomas CM, Simpson PR, Cleary WG, Newman MA, Burcham KW.
    Journal: Mol Gen Genet; 1988 Oct; 214(2):333-42. PubMed ID: 3237209.
    Abstract:
    We have employed a combination of techniques to examine the organization of pea 5S rRNA genes. These include the analysis of length variant interspersion patterns in cosmid clones, sequence analysis, Southern analysis of both conventional gels and field inversion gels and in situ hybridization. From these analyses we conclude that the 5S rRNA genes of pea are arranged in three major tandem arrays which are represented by three large EcoRI fragments and that these correspond to the three sites of in situ hybridization in the haploid pea complement.
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