These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Ouabain induces acetylcholine release from pure cholinergic synaptosomes independently of extracellular calcium concentration.
    Author: Blasi JM, Ceña V, González-García C, Marsal J, Solsona C.
    Journal: Neurochem Res; 1988 Nov; 13(11):1035-41. PubMed ID: 3237303.
    Abstract:
    We have studied the correlation between [3H]ouabain binding sites, (Na+ + K+)ATPase (EC 3.6.1.3) activity and acetylcholine (ACh) release in different subcellular fractions of Torpedo marmorata electric organ (homogenate, synaptosomes, presynaptic plasma membranes). Presynaptic plasma membranes contained the greater number of [3H]ouabain binding sites, in good agreement with the high (Na+ + K+)ATPase activity found in this fraction. Blockade of this enzymatic activity by ouabain dose-dependently induced ACh release from pure cholinergic synaptosomes, either in the presence or absence of extracellular calcium ions. We suggest that one of the mechanisms involved in the ouabain-induced ACh release in the absence of Ca2+o may be an increase in Na+i that could (a) evoke Ca2+ release from internal stores and (b) inhibit ATP-dependent Ca2+ uptake by synaptic vesicles.
    [Abstract] [Full Text] [Related] [New Search]