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  • Title: Visualization and analysis techniques for three dimensional information acquired by confocal microscopy.
    Author: Brakenhoff GJ, van der Voort HT, Baarslag MW, Mans B, Oud JL, Zwart R, van Driel R.
    Journal: Scanning Microsc; 1988 Dec; 2(4):1831-8. PubMed ID: 3238377.
    Abstract:
    Confocal Scanning Laser Microscopy (CSLM) is particularly well suited for the acquisition of 3-dimensional data of microscopic objects. In the CSLM a specific volume in the object is sampled during the imaging process and the result is stored in a digital computer as a three-dimensional memory array. Optimal use of these data requires both the development of effective visual representations as well as analysis methods. In addition to the well known stereoscopic representation method a number of alternatives for various purposes are presented. When rendering in terms of solid-looking or semitransparent objects is required, an algorithm based on a simulated process of excitation and fluorescence is very suitable. Graphic techniques can be used to examine the 3-dimensional shape of surfaces. For (near-)real time applications a representation method should not require extensive previous data-processing or analysis. From the very extensive field of 3-D image analysis two examples are given.
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