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  • Title: [Qualitative difference of mitochondrial subproteoms of brain RPN10- and RPN13-binding proteins].
    Author: Buneeva OA, Kopylov AT, Medvedev AE.
    Journal: Biomed Khim; 2020 Feb; 66(2):138-144. PubMed ID: 32420894.
    Abstract:
    Good evidence exists that the ubiquitin-proteasome system (UPS) plays an important role in degradation of mitochondrial proteins and membrane proteins associated with mitochondria (MAM proteins). Mitochondria contain all components of the ubiquitin-conjugating system, which are necessary for the attachment of ubiquitin molecules to target proteins, subjected to subsequent degradation in proteasomes. An important stage in the delivery of proteins for proteolytic degradation in proteasomes is their interaction with ubiquitin receptors located on the regulatory subunit (19S) of the proteasome: the Rpn10 or Rpn13 subunit. These subunits make basically the same contribution to the subsequent translocation of target proteins to the core part of the proteasome. A comparative study of mouse brain mitochondrial subproteomes bound to Rpn10 and Rpn13 subunits revealed a high specificity of the repertoire of Rpn10 and Rpn13-binding proteins. Moreover, proteins, for which mitochondrial localization or association with mitochondrial membranes was previously shown, prevailed in the case of using the Rpn13 subunit as an affinity ligand (Rpn13-binding proteins). This suggests that Rpn10 and Rpn13 play different roles in the degradation of mitochondrial proteins and MAM. Nakaplivaetsia vse bol'she dannykh v pol'zu togo, chto ubikvitin-proteasomnaia sistema (UPS) igraet vazhnuiu rol' v degradatsii mitokhondrial'nykh belkov i belkov membran, assotsiirovannykh s mitokhondriiami (tak nazyvaemye MAM-belki). V mitokhondriiakh obnaruzheny vse komponenty ubikvitin-kon"iugiruiushcheĭ sistemy, neobkhodimye dlia prisoedineniia molekul ubikvitina k belkam-misheniam, podlezhashchim posleduiushcheĭ degradatsii v proteasomakh. Vazhnym étapom dostavki belkov, podlezhashchikh proteoliticheskoĭ degradatsii v proteasomakh, iavliaetsia ikh vzaimodeĭstvie s retseptorami ubikvitina, raspolozhennymi na reguliatornoĭ subchastitse (19S) proteasomy: sub"edinitseĭ Rpn10 ili Rpn13. Schitaetsia, chto obe sub"edinitsy vnosiat odinakovyĭ vklad v posleduiushchuiu translokatsiiu belkov-misheneĭ v korovuiu chast' proteasomy. Sravnitel'noe issledovanie mitokhondrial'nykh subproteomov mozga mysheĭ, sviazyvaiushchikhsia s sub"edinitsami Rpn10 i Rpn13, vyiavilo vysokuiu spetsifichnost' repertuara Rpn10- i Rpn13-sviazyvaiushchikh belkov. Pri étom kolichestvo belkov, dlia kotorykh ranee byla pokazana mitokhondrial'naia lokalizatsiia ili assotsiatsiia s mitokhondrial'nymi membranami, preobladalo pri ispol'zovanii v kachestve affinnogo liganda sub"edinitsy Rpn13. Vse éto svidetel'stvuet v pol'zu togo, chto Rpn10 i Rpn13 igraiut raznye roli v degradatsii belkov mitokhondriĭ i MAM.
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