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  • Title: Dual-color STED super-resolution microscope using a single laser source.
    Author: Wang J, Zhang J, Wang L, Gao X, Shao Y, Liu L, Yang Z, Yan W, Qu J.
    Journal: J Biophotonics; 2020 Aug; 13(8):e202000057. PubMed ID: 32421923.
    Abstract:
    STED (stimulated emission depletion) microscopy is one of the most promising super-resolution fluorescence microscopies，due to its fast imaging and ultra-high resolution. In this paper, we present a dual-color STED microscope with a single laser source. Polarization beam splitters are used to separate the output from a supercontinuum laser source into four laser beams, including two excitation beams (488, 635 nm) and two depletion beams (592, 775 nm). These four laser beams are then used to build a low cost dual-color STED system to achieve a spatial resolution of 75 nm in cell samples.
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