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  • Title: Determination of cortisol in human plasma by stable isotope dilution mass spectrometry.
    Author: Kasuya Y, Furuta T, Hirota N.
    Journal: Biomed Environ Mass Spectrom; 1988 Oct; 16(1-12):309-11. PubMed ID: 3242683.
    Abstract:
    Cortisol selectively labelled with 2H at the 19-methyl and the C-1 position (cortisol-d5) was synthesized and used as an internal standard in stable isotope dilution mass spectrometry in order to determine cortisol in human plasma. A capillary gas chromatographic/mass spectrometric method provided a sensitive and reliable technique with good accuracy, precision and reproducibility without complex corrections for contributions by using cortisol-d5 as an internal standard. For calculation of plasma cortisol, peak areas were measured by selected ion monitoring on the characteristic fragment ions of the dimethoxime tri(trimethylsilyl) derivatives of cortisol and cortisol-d5 (m/z 605 and 610, respectively). The sensitivity of the gas chromatographic/mass spectrometric assay was 1.02 ng per injection, with a signal-to-noise ratio of about 6. The inter- and intra-assay coefficients of variation for plasma sample were 3.07% and 1.77%, respectively.
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