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  • Title: Acid phosphatase typing for breeding nematode-resistant tomatoes by isoelectric focusing with an ultranarrow immobilized pH gradient.
    Author: Günther S, Postel W, Wiering H, Görg A.
    Journal: Electrophoresis; 1988 Sep; 9(9):618-20. PubMed ID: 3243260.
    Abstract:
    The genetic variants of tomato acid phosphatase (Aps-1) systems have been analyzed by isoelectric focusing in immobilized pH gradients (IPG). By using an ultranarrow pH 4.25-4.55 IPG gel, the two genotypes Aps-1(1) and Aps-1+, differentiating tomato variants into nematode-resistant or nematode-susceptible plants, are separated into two sharp zones over a distance of 2.5 cm with isoelectric points of 4.37 and 4.43, respectively. Under these conditions, silver staining of the Aps-1 variants proved to be superior to enzyme staining. By applying more than 50 samples on one IPG gel, this method proved to be a powerful tool for reliable tomato nematode resistance screening.
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