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  • Title: 5'-Hydroxymethyl fluorescein: A colorimetric chemosensor for naked-eye sensing of cyanide ion in a biological fluid.
    Author: Rathod RV, Bera S, Mondal D.
    Journal: Spectrochim Acta A Mol Biomol Spectrosc; 2020 Sep 05; 238():118419. PubMed ID: 32438290.
    Abstract:
    A two-step synthetic method to prepare a highly sensitive and selective chemosensor 5'-hydroxymethyl fluorescein (5'-HMF) is described herein. This sensor was explored as a colorimetric sensor for naked-eye detection of cyanide ion in the biological fluid as well as in organic and aqueous media. The addition of cyanide ion to 5'-HMF resulted in a rapid change in color in aqueous medium from light green to dark fluorescent green, and in acetonitrile from light pink to purple. A significant bathochromic shift in the absorption spectra enables cyanide ion to be detected by naked eyes in water and acetonitrile without any interference of the competing anions such as, AcO-, F- and SCN- in aqueous solution. Using the 1HNMR titration experiments and Job's plot from absorbance spectroscopy, the interaction of CN- ion with 5'-HMF has been investigated and binding stoichiometry was found to be 1:2 (5'-HMF to CN-). The limit of detection (LOD) of the sensor for CN- was 3.68 μM in water with a linearity (R2 = 0.9923) in the range of 0.50 to 30.0 μM concentration assuming 1:2 (5'-HMF to CN-) binding stoichiometry. In addition, the sensor 5'-HMF sensed the CN- ion in human saliva with the LOD as 7.0 μM in aq. medium.
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