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  • Title: An innovative alkaline protease-based pretreatment approach for enhanced short-chain fatty acids production via a short-term anaerobic fermentation of waste activated sludge.
    Author: Pang H, Pan X, Li L, He J, Zheng Y, Qu F, Ma Y, Cui B, Nan J, Liu Y.
    Journal: Bioresour Technol; 2020 Sep; 312():123397. PubMed ID: 32526667.
    Abstract:
    This study reported a novel pretreatment approach with combination of alkaline protease (AP) and pH 10 for enhancing short-chain fatty acids (SCFAs) production from waste activated sludge (WAS). Through the AP-based pretreatment, WAS flocs were disintegrated with cell lysis, leading to release of biodegradable organic matters. At the external AP dosage of 5%, SCOD of 5363.7 mg/L (SCOD/TCOD = 32.5%) was achievable after 2-h pretreatment. More than 66% of SCOD was composed of proteins and carbohydrates. Considerable SCFAs of 607 mg COD/g VSS was produced over a short-term anaerobic fermentation of 3 days, which was 5.4 times higher than that in the control. Acetic and propionic acids accounted for 74.1% of the SCFAs. The AP-based approach increased endogenous protease and α-glucosidase activities, facilitating biodegradation of dissolved organic matters and SCFAs production. Such approach is promising for WAS disposal and carbon recovery, the produced SCFAs might supply 60% of carbon gap in wastewater.
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