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  • Title: Fracture-flip: new high-resolution images of cell surfaces after carbon stabilization of freeze-fractured membranes.
    Author: Andersson Forsman C, Pinto da Silva P.
    Journal: J Cell Sci; 1988 Aug; 90 ( Pt 4)():531-41. PubMed ID: 3253295.
    Abstract:
    We introduce fracture-flip, a method of obtaining high-resolution views of membrane surfaces. The method, a corollary of label-fracture, is based on the stabilization of the exoplasmic halves of membranes by carbon evaporation. Inversion of these casts followed by Pt/C imaging leads to new views of the cell surface at macromolecular resolution.
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