These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Granulocyte-monocyte progenitor cells from human peripheral blood: modulation of growth in vitro by T lymphocytes and monocytes.
    Author: Dooley DC, Law P, Alsop P.
    Journal: Int J Cell Cloning; 1988 Jan; 6(1):45-59. PubMed ID: 3257516.
    Abstract:
    Cell fractionation techniques have been used for the purification and characterization of hematopoietic cells present in peripheral blood and bone marrow. Following fractionation, the distribution of hematopoietic cells is frequently determined by the granulocyte-monocyte colony-forming unit (CFU-gm) assay. In this study, we questioned whether the purification process itself altered the sensitivity of the CFU-gm assay through changes in the accessory cell populations. Experiments showed that following T cell depletion, the cloning efficiency of CFU-gm was suboptimal, since the addition of autologous T lymphocytes was stimulatory, even when the concentration of conditioned medium was optimal. In contrast, growth of CFU-gm was inhibited by monocytes, both in the presence and absence of T cells. Sensitivity to monocyte-derived inhibition occurred at significantly lower monocyte concentrations when T cells were present. Thus, stem cell purification techniques which deplete T lymphocytes or enrich monocytes have an adverse effect on the cloning efficiency of peripheral blood CFU-gm. This study presents culture techniques capable of circumventing these problems.
    [Abstract] [Full Text] [Related] [New Search]