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  • Title: Measurement of intracellular Ca2+ in the bullfrog sympathetic ganglion cells using fura-2 fluorescence.
    Author: Nohmi M, Kuba K, Ogura A, Kudo Y.
    Journal: Brain Res; 1988 Jan 12; 438(1-2):175-81. PubMed ID: 3257890.
    Abstract:
    The intracellular free Ca2+ concentration ([Ca2+]i) of the bullfrog sympathetic ganglion cell was measured with fura-2 fluorescence under various conditions, and compared with changes in membrane potential recorded with an intracellular electrode. The [Ca2+]i was 109 nM on average under the resting condition and increased by raising the extracellular K+, stimulating repetitively the pre- or post-ganglionic nerve, or by applying acetylcholine or muscarine. Since all these procedures depolarized the cell membrane, most of the rise in [Ca2+]i could be the result of opening of voltage-dependent Ca2+ channels. However, Ca2+ entries through nicotinic acetylcholine receptor channels and the channel activated by the muscarinic acetylcholine receptor were also indicated by considering the threshold for the opening of voltage-dependent Ca2+ channels (for both entries) or a limited number of the cells showing the latter response.
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