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  • Title: [Relationship between gene polymorphism at rs2228055 locus in the exon region of interleukin-10 receptor A and susceptibility to food allergy in children].
    Author: Sun JB, Li ZL.
    Journal: Zhonghua Er Ke Za Zhi; 2020 Jul 02; 58(7):559-563. PubMed ID: 32605339.
    Abstract:
    Objective: To investigate the relationship between gene polymorphism of rs2228055 locus in the exon region of interleukin-10 receptor A (IL-10RA) and susceptibility to food allergy in children. Methods: This was a case-control study. The food allergy group had 150 children who were diagnosed with food allergy in the Pediatric Food Allergy Clinic of Peking University Third Hospital from August 1, 2017 to November 30, 2018. Another 150 healthy children attended Child Health and Development Center in the same hospital were selected as control group. The genotypes of rs2228055 locus in both groups were detected by PCR re-sequencing. And the genotypes and allele frequencies of rs2228055 locus were compared between these two groups, as well as between food allergy children with positive and negative allergen specific IgE, and between those with and without involvement of different organs. Using the computer virtual mutation to stimulate the changes of amino acid caused by change of rs2228055 locus allele, to analyze the effect of amino acid changes on the structure of IL-10RA. The chi-square test was used for comparison between groups. Results: (1) There were 92 males and 58 females in food allergy group, and 86 males and 64 females in control group, without any statistically significant difference (χ(2)=0.497, P=0.481). The ages of the two groups were 4.2 (0.1-15.0) and 8.0 (0.1-14.0) years old, respectively, the difference was statistically significant (Z=-6.109, P<0.01). (2) The genotype frequencies of rs2228055 locus in the food allergy group and the control group were as follows: AA accounted for 73 (48.7%) and 98 (65.3%), AG accounted for 62 (41.3%) and 42 (28.0%), and GG accounted for 15 (10.0%) and 10 (6.7%), respectively. The allele frequencies in the two groups were as follows: 208 (69.3%) and 238 (79.3%) for A, 92 (30.7%) and 62 (20.7%) for G, respectively. AG and GG genotype frequency and the allele G frequency in food allergy group were significantly higher than that in control group (χ(2)=8.501 and 7.862, P=0.014 and 0.005, respectively). (3) There were no significant differences in genotype frequencies and allele frequencies of rs2228055 locus of allergen-specific IgE-positive and negative food allergy children (all P>0.05). (4) There were no significant differences in genotype frequencies and allele frequencies of rs2228055 locus in the manifestations of skin, digestive system and respiratory system in food allergy children (all P>0.05). (5) The computer virtual mutation showed that the mutation energy was -0.08 without any increase in the stability of IL-10RA when the amino acid encoded by rs2228055 locus was changed from isoleucine to valine. Conclusions: The frequencies of genotype AG, GG and allele G of rs2228055 locus in the IL-10RA exon region in food allergy children are higher than that in non-allergic children, and those with the G allele are more likely to develop food allergy. 目的： 探讨白细胞介素10受体A（IL-10RA）外显子区域rs2228055位点基因多态性与儿童食物过敏易感性的关系。 方法： 病例对照研究。选择2017年8月1日至2018年11月30日在北京大学第三医院儿科食物过敏门诊被诊断为食物过敏的150例患儿作为食物过敏组，选择同期在北京大学第三医院儿童健康发展中心健康体检的150名儿童作为对照组。用PCR重测序的方法检测两组儿童rs2228055位点基因型，比较两组儿童该位点基因型及等位基因频率，同时比较该位点基因型及等位基因频率在过敏原特异性IgE阳性与IgE阴性的食物过敏患儿、在食物过敏患儿不同器官系统表现中的分布。采用计算机虚拟突变rs2228055位点等位基因改变后引起的氨基酸改变，分析氨基酸改变后对IL-10RA结构的影响。组间比较采用χ(2)检验。 结果： （1）食物过敏组男92例、女58例，对照组男86名、女64名，组间比较差异无统计学意义（χ(2)=0.497，P=0.481），食物过敏组和对照组年龄分别为4.2（0.1~15.0）岁、8.0（0.1~14.0）岁，组间比较差异有统计学意义（Z=-6.109，P<0.01）。（2） rs2228055位点基因型在食物过敏组与对照组分别为AA 73例（48.7%）、98例（65.3%）；AG 62例（41.3%）、42例（28.0%）；GG 15例（10.0%）、10例（6.7%）；等位基因频率在两组分别为A 208（69.3%）、238（79.3%）；G 92（30.7%）、62（20.7%），食物过敏组患儿AG及GG基因型及等位基因G频率高于对照组儿童（χ(2)=8.501、7.862，P=0.014、0.005）。（3）rs2228055位点基因型及等位基因频率在过敏原特异性IgE阳性和阴性组食物过敏患儿中分布差异无统计学意义（P均>0.05）。（4）rs2228055位点基因型及等位基因频率在有皮肤表现和无皮肤表现、有消化系统表现和无消化系统表现、有呼吸系统表现和无呼吸系统表现的食物过敏患儿中分布差异无统计学意义（P均>0.05）。（5）计算机虚拟突变发现，当rs2228055位点所编码的氨基酸由异亮氨酸转变为缬氨酸后，其突变能为-0.08，IL-10RA的稳定性没有增加。 结论： 食物过敏患儿IL-10RA外显子区域rs2228055位点基因型AG、GG及等位基因G频率高于对照组儿童，携带等位基因G的儿童更容易发生食物过敏。.
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