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  • Title: Enzymatic Quantification of Liver Lipids After Folch Extraction.
    Author: Minniti ME, Ahmed O, Pedrelli M.
    Journal: Methods Mol Biol; 2020; 2164():101-108. PubMed ID: 32607887.
    Abstract:
    The determination of the lipid content in liver offers the potential to investigate metabolic alterations in different research contexts. Here, we describe a method to determine cholesterol, triglycerides, and phospholipids in liver samples based on total lipid isolation by a 2:1 chloroform-methanol mixture (Folch extraction) and specific enzymatic colorimetric microassays in plate.
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