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  • Title: Rabbit antisera to enterobacteriaceae isolated from HLA-B27 positive patients with ankylosing spondylitis (AS) are lytic for the mononuclear cells of AS patients.
    Author: Beukelman CJ, van Leeuwen A, Aerts PC, van Kregten E, van Bree S, van Rood JJ, Willers JM, van Dijk H.
    Journal: Br J Rheumatol; 1988; 27 Suppl 2():58-60. PubMed ID: 3261191.
    Abstract:
    Vaccines prepared from Gram-negative bacteria isolated from the stools of HLA-B27 positive AS patients were used to immunize rabbits. Three of the sera obtained were lytic in vitro for the mononuclear cells of HLA-B27 positive AS patients. One of these sera discriminated between AS patients and healthy HLA-B27 positive individuals. Cytolysis was determined in an automated, non-radioactive assay based on the release of carboxyfluorescein diacetate and the incorporation of propidium iodide.
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