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  • Title: The anti-caries efficacy of three fluoride compounds at increasing maturation of a microcosm biofilm.
    Author: Ayoub HM, Gregory RL, Tang Q, Lippert F.
    Journal: Arch Oral Biol; 2020 Sep; 117():104781. PubMed ID: 32622258.
    Abstract:
    OBJECTIVE: To explore the anti-caries efficacy of three fluoride compounds at increasing maturation of a microcosm biofilm. DESIGN: Microcosm biofilm, obtained from saliva collected from three donors (IRB #1406440799), was grown on enamel samples (n = 18/group) for 24-h (Brain Heart Infusion; 0.2 % sucrose). Then, pH cycling model started. Three maturations were explored (4d, 8d, and 12d). The pH cycling consisted of daily 2 × 5 min treatments (NaF, SnF2, AmF: 287.5 ppm F, and de-ionized water [DIW]), 4 × 10 min remineralization (BHI, no sucrose, pH 7.0), and 3 × 2:15 h demineralization (BHI, 1% sucrose, pH 4.5). We analyzed the enamel (surface microhardness [VHNchange], integrated mineral loss [ΔZ], lesion depth [L]), and the biofilm (viability [log10 CFU/mL], lactic acid production [LDH], and exopolysaccharide [EPS] amount). Data were analyzed using two-way ANOVA (p = 0.05). RESULTS: The interaction between tested variables was significant for VHNchange, viability, LDH, EPS (p = 0.0354, p = 0.0001, p < 0.0001, p < 0.0001), but not for L (p = 0.2412) or ΔZ (p = 0.6811). LDH and EPS analyses exhibited more tolerance of mature biofilm against NaF (LDH and EPS p < 0.0001); NaF-treated groups demonstrated significantly lower results than the control in the 12d group. The effect of SnF2 and AmF continued over time. VHNchange, L, and ΔZ: The effect of SnF2 and AmF was higher than NaF and DIW. L and ΔZ did not result in significant differences over time (all treatments). Within each maturation, fluoride compounds demonstrated statistically significantly lower L and ΔZ values than DIW. CONCLUSIONS: Biofilm's maturation may influence the selection of fluoride compounds to achieve an optimum cariostatic effect.
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