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  • Title: Alterations of cellular lipids in rat thymocytes during cell cycle progression.
    Author: Gross G, Danzl M, Fischer W, Brand K.
    Journal: Biochim Biophys Acta; 1988 Sep 23; 962(2):220-6. PubMed ID: 3262373.
    Abstract:
    This study provides information on the lipid content and composition of rat thymocytes during cell cycle progression. Cultured rat thymocytes were stimulated by concanavalin A and interleukin-2 to enter the cell cycle and to proliferate. At indicated intervals during a 96 h culture, cells were analyzed for lipid content and lipid synthesis. A growth-related 3.8-fold increase in the cellular cholesterol and a 2.8-fold increase in the phospholipid content was observed after 48 h of culture. Cholesterol synthesis, measured by [14C]acetate incorporation, increased 34-fold, whereas the synthesis of the other lipids was enhanced only 15-fold. Concerning the percentage distribution of the total fatty acids, arachidonic acid showed a significant decline between 24 and 72 h of culture, being mainly caused by the release of arachidonic acid from phosphatidylcholine. Addition of actinomycin D, an inhibitor of DNA replication, prevented the decline of cellular arachidonic acid content in a dose-dependent manner. These data suggest that both the pronounced rise of the cholesterol content as well as the decline of arachidonic acid content are closely linked to cell proliferation.
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