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  • Title: [Intracellular localization of casein kinase of type II in oocytes of Rana temporaria].
    Author: Kandror KV, Beniumov AO, Stepanov AS.
    Journal: Mol Biol (Mosk); 1988; 22(4):1097-107. PubMed ID: 3263565.
    Abstract:
    Casein kinase II has been isolated from ribosome-free extracts of Rana temporaria oocytes by chromatography on heparin-sepharose, phosphocellulose and mono Q. It consists of different subunits with molecular weights 43, 41 and 29 Kda. Protein kinase has been labelled in vitro with 125I and injected back into amphibian oocytes. With the help of radioautography it is shown that this enzyme forms in the oocyte cytoplasm a wide concentric ring around the nucleus. Part of the labelled casein kinase II is associated with free cytoplasmic informosomes.
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