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  • Title: Biodegradable film based on pectin/Nano-clay/methylene blue: Structural and physical properties and sensing ability for measurement of vitamin C.
    Author: Pirsa S.
    Journal: Int J Biol Macromol; 2020 Nov 15; 163():666-675. PubMed ID: 32652151.
    Abstract:
    In this study, biodegradable film based on pectin modified with Nanoclay and methylene blue was prepared. The Physicomechanical properties of the prepared film, including: thickness, water solubility, water vapor permeability (WVP), antioxidant activity and color properties were investigated. Scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Differential scanning calorimetry (DSC) and texture analysis techniques were used to investigate the physical and mechanical properties of the films. The optimum Pec/Clay/MB film was used as a colorimeter sensor to detect and measure vitamin C. The results showed that the addition of Nanoclay to the film increased the thickness and decreased the solubility of water and reduced the WVP. The addition of methylene blue had no significant effect on the thickness, but increased water solubility and increased antioxidant activity. The addition of Nanoclay increased the tensile strength (TS) of the film, but decreased its elongation at break (EB). The addition of Nanoclay increased the melting temperature of the film and decreased its crystallization temperature. In the SEM images, the clay particles at 50-100 nm in the film structure were characterized. Nanoclay and methylene blue reduced the surface cleavage of the film. Pec/Clay/MB film color was changed from blue to colorless in the presence of vitamin C. The linear range of vitamin C measurement was 10 to 500 and the detection limit was 10. By detecting the color changes of the film in the presence of vitamin C, the amount of this vitamin was measured in orange, kiwi and tangerine juice. The accuracy of the data was evaluated by the titration method. The sensor accuracy was obtained over 90%.
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