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  • Title: Analysis of metabolically labeled inositol phosphate messengers by NMR.
    Author: Puschmann R, Harmel RK, Fiedler D.
    Journal: Methods Enzymol; 2020; 641():35-52. PubMed ID: 32713530.
    Abstract:
    Inositol phosphates (InsPs) are an important group of eukaryotic messengers and mediate a wide range of processes. To elucidate the biological functions of these molecules, robust techniques to characterize inositol phosphate metabolism at the cellular level are highly sought after. This chapter provides a detailed protocol for the preparation of 13C-labeled myo-inositol, its use for metabolic labeling of mammalian and yeast cells, and the quantitative analysis of intracellular InsP pools from cell extracts using NMR spectroscopy.
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