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  • Title: A cell type-specific enhancer drives expression of the chick muscle acetylcholine receptor alpha-subunit gene.
    Author: Wang Y, Xu HP, Wang XM, Ballivet M, Schmidt J.
    Journal: Neuron; 1988 Aug; 1(6):527-34. PubMed ID: 3272179.
    Abstract:
    The regulation of acetylcholine receptor alpha-subunit gene expression was analyzed by transient expression assays. Using rabbit beta-globin cDNA as a reporter gene, we have confirmed that the 5'-flanking sequence of the chicken acetylcholine receptor alpha-subunit gene directs specific expression in differentiated C2C12 cells, a mouse muscle cell line, but not in undifferentiated C2C12 cells and mouse 3T3 fibroblasts. Testing chimeric plasmids containing Bal31 deletion mutants of the alpha-subunit gene upstream sequence, we found the -116 to -81 region of the alpha-subunit to be responsible for tissue- and stage-specific expression. This 36 bp fragment stimulates the activity of both alpha-subunit and SV40 promoters in a distance- and orientation-independent manner, thus fulfilling the criteria of an enhancer.
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