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  • Title: Development of indirect ELISA for the detection of canine adenovirus type 2 antibodies in dog sera.
    Author: Yang DK, Kim HH, Lee S, Oh D, Yoo JY, Hyun BH.
    Journal: J Vet Sci; 2020 Jul; 21(4):e63. PubMed ID: 32735100.
    Abstract:
    BACKGROUND: Canine adenovirus type 2 (CAV-2) induces infectious laryngotracheitis in members of the family Canidae, including dogs. To date, no ELISA kits specific for CAV-2 antibody have been commercialized for dogs in Korea. OBJECTIVES: We aimed to develop new indirect enzyme-linked immunosorbent assay (I-ELISA) to perform rapid, accurate serological surveys of CAV-2 in dog serum samples. METHODS: In total, 165 serum samples were collected from dogs residing in Chungbuk and Gyeongbuk provinces between 2016 and 2018. The Korean CAV-2, named the APQA1701-40P strain, was propagated in Madin-Darby canine kidney cells and purified in an anion-exchange chromatography column for use as an antigen for I-ELISA. The virus-neutralizing antibody titers of CAV-2 in the dog sera were measured by virus neutralization (VN) test. RESULTS: We compared the results obtained between the VN and new I-ELISA tests. The sensitivity, specificity, and accuracy of new I-ELISA were 98.6%, 86.4% and 97.0% compared with VN test, respectively. New I-ELISA was significantly correlated with VN (r = 0.91). CONCLUSIONS: These results indicate that new I-ELISA is useful for sero-surveillance of CAV-2 in dog serum.
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