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  • Title: Acetone, butanol, and ethanol production from puerariae slag hydrolysate through ultrasound-assisted dilute acid by Clostridium beijerinckii YBS3.
    Author: Zhou ZY, Yang ST, Moore CD, Zhang QH, Peng SY, Li HG.
    Journal: Bioresour Technol; 2020 Nov; 316():123899. PubMed ID: 32739577.
    Abstract:
    In this study, puerariae slag (PS) was evaluated as a renewable raw material for acetone-butanol-ethanol (ABE) fermentation. To accelerate the hydrolysis of PS, the method of ultrasound-assisted dilute acid hydrolysis (UAAH) was used. With this effort, 0.69 g reducing sugar was obtained from 1 g raw material under the optimal pretreatment condition. Subsequently, the butanol and total solvent production of 8.79 ± 0.16 g/L and 12.32 ± 0.26 g/L were obtained from the non-detoxified diluted hydrolysate, and the yield and productivity of butanol were 0.19 g/g and 0.12 g/L/h, respectively. Additionally, the changes in the structure of PS after different pretreatment methods were observed using SEM and FT-IR. UAAH resulted in more severe and distinct damage to the dense structure of PS. This study suggests that the UAAH is an attainable but effective pretreatment method, thereby is a promising technique for lignocellulose hydrolysis and improve butanol production.
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