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  • Title: Discrimination of B, T and null lymphocytes by esterase cytochemistry.
    Author: Higgy KE, Burns GF, Hayhoe FG.
    Journal: Scand J Haematol; 1977 May; 18(5):437-48. PubMed ID: 327533.
    Abstract:
    A technique has been devised which optimally demonstrates non-specific esterase activity in human blood lymphocytes. The reaction is carried out on smears fixed with formalin vapour, using alpha-naphthol butyrate as substrate at pH 8 and at a low concentration for a short incubation period. The pattern of esterase activity revealed by this method provides a discriminating marker for mature T-lymphocytes, which show dense, localised, dot-like positivity, and a probable marker for 'null' cells in the form of scattered granular positivity. B cells appear to be negative. Monocytes show a clearly different pattern of granular positivity.
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