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  • Title: Cloning of the Escherichia coli K-12 hemB gene.
    Author: Li JM, Umanoff H, Proenca R, Russell CS, Cosloy SD.
    Journal: J Bacteriol; 1988 Feb; 170(2):1021-5. PubMed ID: 3276659.
    Abstract:
    An Escherichia coli heme-requiring, heme-permeable mutant had no detectable 5-aminolevulinate dehydratase or porphobilinogen deaminase activities. The gene which complemented this mutation was cloned to a high-copy-number plasmid, and porphobilinogen deaminase activity was restored to normal levels, but the synthesis of 5-aminolevulinate dehydratase increased 20- to 30-fold. A maxicell procedure confirmed that the gene cloned was hemB.
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