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  • Title: [The nitroblue tetrazolium reduction test in evaluating the function of the circulating leukocytes in mice with experimental malaria].
    Author: Gilpin-Dzhekson E, Shadrin BP.
    Journal: Biull Eksp Biol Med; 1988 Feb; 105(2):166-8. PubMed ID: 3280042.
    Abstract:
    NBT-test for circulating neutrophils and monocytes in the blood of mice inoculated with Plasmodium berghei, strain N or LNK-65, have been performed. Within the first 24 h of the infection, before the onset of the recordable parasitemia or in the course of the subsequent six days (depending of the strain used for inoculation) a 50-100% reduction in NBT-positive cells was observed. This demonstrates the ability of malaria parasite to suppress the oxygen-dependent enzyme system in circulating phagocytes, neutrophils and monocytes of the host blood. The results of NBT-test could be utilized for the investigation of immunological disorders and also for the differential diagnosis of malarial infection.
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