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  • Title: [Evaluation of the vitamin D activity of yeasts with altered sterol metabolism].
    Author: Mikhaĭlova NP, Valinietse MIu, Bauman VK, V'iunov KA.
    Journal: Prikl Biokhim Mikrobiol; 1988; 24(1):98-101. PubMed ID: 3285341.
    Abstract:
    The sterol content of Saccharomyces strains with altered ergosterol metabolism was studied by UV-spectrophotometry, thin-layer chromatography and chromatographic mass-spectroscopy. A technique for estimation of D-vitamin activity of the yeast strains is proposed. The irradiated biomass of the strains accumulated ergosta-5,7-dien-3 beta-ol and also cholesta-5,7,24-trien-3 beta-ol and cholesta-5,7,22,24-tetraen-3 beta-ol is characterized by high antirachitic activity.
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