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  • Title: [Immunoenzyme test system based on antibody F(ab)2-fragments for detecting the NP protein of the influenza virus].
    Author: Leonov SV, Khristova ML, Sokolova MV, Dönner L, Kharitonenkov IG.
    Journal: Vopr Virusol; 1988; 33(1):18-21. PubMed ID: 3285591.
    Abstract:
    A test system has been proposed for the detection of influenza A virus NP protein by solid-phase enzyme immunoassay in a modified system of "double" antibodies: the primary antibodies were F(ab)2-fragments of immunoglobulin class G isolated from rabbit antiserum specific for NP protein which was also used as the source of secondary antibodies. The antigen-bound antibodies were detected by protein A (from St. aureus) conjugate with horseradish peroxidase. The high specificity and sensitivity of the test system (0.3 ng/ml NP protein) and a minimal level of nonspecific reactions were demonstrated. The advantages of this method are discussed.
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