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  • Title: Cloning of the gene, speB, for streptococcal pyrogenic exotoxin type B in Escherichia coli.
    Author: Bohach GA, Hauser AR, Schlievert PM.
    Journal: Infect Immun; 1988 Jun; 56(6):1665-7. PubMed ID: 3286506.
    Abstract:
    The structural gene encoding streptococcal pyrogenic exotoxin type B, designated speB, was cloned in Escherichia coli and localized onto a 4.5-kilobase BamHI-BglII DNA fragment. Streptococcal pyrogenic exotoxin type B, partially purified from E. coli clones, was immunologically related to streptococcus-derived toxin. Also, toxin derived from either E. coli or Streptococcus pyogenes had similar lymphocyte mitogenic activity and molecular weight (29,300) and displayed comparable microheterogeneity when evaluated by isoelectric focusing.
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