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  • Title: Characterization of a phytochelatin synthase gene from Ipomoea pes-caprae involved in cadmium tolerance and accumulation in yeast and plants.
    Author: Su H, Zou T, Lin R, Zheng J, Jian S, Zhang M.
    Journal: Plant Physiol Biochem; 2020 Oct; 155():743-755. PubMed ID: 32866789.
    Abstract:
    Phytochelatin synthases (PCSs) play a crucial part in heavy metal tolerance in plants via the synthesis of phytochelatins (PCs), which can chelate heavy metals (HMs) in the vacuole and decrease cell damage. Plant PCSs are commonly designated as key genes for phytoremediation. In this study, we identified a PCS gene (IpPCS1) from Ipomoea pes-caprae and investigated its role in regulating cadmium (Cd) tolerance and accumulation. The expression of a truncated IpPCS1t in yeast could complement the Cd-sensitive phenotype of the ycf1Δ mutant strain, as well as improve the Cd tolerance of the wild-type yeast strain, while promoting Cd accumulation in the yeast cells. The expression of IpPCS1 was induced in I. pes-caprae plants under Cd treatment. Compared with IpPCS1, the lack of a C-terminal in IpPCS1t did not affect its Cd tolerance, but might restrict the zinc (Zn) detoxification in yeast. The overexpression of IpPCS1t in Arabidopsis could improve the Cd tolerance slightly and had little impact on Cd accumulation in transgenic plant. Our results indicated that IpPCS1 has certain potential application value in Cd tolerance and detoxification, therefore provides a useful genetic resource for enhancing Cd tolerance and improving the Cd phytoremediation capacity of plants or organisms. In addition, our research is the first time to discover a new possible Cd activation site in the C-terminal of IpPCS1.
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