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  • Title: Silver nanoclusters-based fluorescent biosensing strategy for determination of mucin 1: Combination of exonuclease I-assisted target recycling and graphene oxide-assisted hybridization chain reaction.
    Author: Wu H, Wu J, Liu Y, Wang H, Zou P.
    Journal: Anal Chim Acta; 2020 Sep 08; 1129():40-48. PubMed ID: 32891389.
    Abstract:
    A novel label-free fluorescent biosensing strategy was described for the sensitive detection of mucin 1 (MUC1). It consisted of an M-shaped aptamer probe for exonuclease I (Exo I)-assisted target recycling (EATR) amplification, and two AgNCs-hairpin probes for graphene oxide (GO)-assisted hybridization chain reaction (HCR) amplification. Based on the specificity of aptamer-target recognition, the addition of MUC1 caused a conformational change in the M-shaped aptamer probe, which was split into a MUC1-P3 complex and a P1-P2 duplex. Exo I then catalyzed the cleavage of aptamer sequence P3 from the MUC1-P3 complex and released the target MUC1. The released target MUC1 was free to bind with a new M-shaped probe to perform EATR amplification. Furthermore, the P1-P2 duplex with three single-stranded arms can act as a primer to initiate HCR between hairpin probes AgNCs-H1 and AgNCs-H2. In the process of HCR, two AgNCs-hairpins were autonomously cross-opened, generating long linear double-stranded nanowires containing large numbers of AgNCs. These nanowires cannot be quenched by GO due to the weak affinity between the long double-stranded DNA and GO, thereby retaining a strong fluorescent signal indicative of the concentration of MUC1. With these designs, in addition to an extremely low detection limit of 0.36 fg mL-1, the method exhibited an acceptable linear response to detect MUC1 from 1 fg mL-1 to 1 ng mL-1. Additionally, this method could be exerted with a high degree of success to detect MUC1 in diluted human serum with satisfactory results.
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