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Title: Enzyme immunoassay of cortisol in human plasma using penicillinase as label. Author: Shrivastav TG, Kumari GL, Rao PN. Journal: Clin Chim Acta; 1988 May 13; 174(1):83-91. PubMed ID: 3289798. Abstract: An enzyme immunoassay for cortisol in human plasma using an antiserum raised against cortisol-3-O-carboxy-methyloxime bovine serum albumin and cortisol-21-hemisuccinate conjugated to penicillinase as tracer is described. Although employing immunoassay plates for separation of antigen-antibody complex from the free components was less time consuming, the slope and sensitivity of the standard curve were improved by the addition of goat anti-rabbit gamma globulin for precipitating the complex. There was good correlation between radioimmunoassay and enzyme immunoassay results obtained for cortisol levels present in normal human plasma.[Abstract] [Full Text] [Related] [New Search]