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  • Title: Interferon-γ and IL-10 Release Assay for Patients with Ocular Toxoplasmosis.
    Author: Rudzinski M, Pardini L, Bernstein M, Moré G, Khoury M, Duarte SC, Argüelles C, Reina S, Oubiña JR.
    Journal: Am J Trop Med Hyg; 2020 Dec; 103(6):2239-2243. PubMed ID: 32901605.
    Abstract:
    Peripheral blood mononuclear cells (PBMC) from patients with ocular toxoplasmosis were challenged with total antigens from Toxoplasma gondii lysate (TATL) in a cytokine release assay (CRA), run during the inactive period of the disease. Increased interferon gamma (IFN-γ) levels were detected after PBMC stimulation with either ME49 reference strain (P = 0.0015) or local TgCkAr-11-9 isolate (P = 0.0012), as compared with those recorded under basal conditions. TATL from TgCkAr11-9 isolate induced a higher release of IFN-γ than ME49 strain in CRA from all tested patients (P = 0.02). The median value of IFN-γ release on TgCkAr-11-9 stimulation (26.03 pg/mL) allowed the classification of patients into high- or low-/non-IFN-γ releasers. Clinical correlations were established with both groups. The results obtained in this study suggest the need to include local strains when performing CRA with TATL.
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