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  • Title: Use of immunosorbent-purified antigens of Fasciola hepatica in enzyme immunoassays.
    Author: Trudgett A, Anderson A, Hanna RE.
    Journal: Res Vet Sci; 1988 Mar; 44(2):262-3. PubMed ID: 3291031.
    Abstract:
    A monoclonal antibody specific for the T1 tegumental antigen of Fasciola hepatica was used as a solid-phase immunosorbent for the purification of T1 antigen from homogenised mature F hepatica. Material fractionated by this technique was successfully used in enzyme-linked immunoassays to detect antibodies to F hepatica in sera from sheep and cattle. Species differences in response to infection by F hepatica were demonstrated.
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