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Title: [Effect of electroacupuncture of "biaoben acupoint combination" on central sensitivity of cholecystokinin in obese rats with insulin resistance].
Author: Song AQ, Zhang YP, Yao M, Liang FX.
Journal: Zhongguo Zhen Jiu; 2020 Sep 12; 40(9):969-75. PubMed ID: 32959593.
Abstract:
OBJECTIVE: To observe the effect of electroacupuncture (EA) of "biaoben acupoint combination" on appetite, body fat, insulin sensitivity and central sensitivity of cholecystokinin (CCK) in obese rats with insulin resistance (IR), and to explore the mechanism of EA on improving obesity with insulin resistance. METHODS: Among the fifty 8-week-old healthy SPF male Wistar rats, 10 rats were randomly selected and fed with normal diet; after 8 weeks, 8 rats were randomly selected as a normal group. The remaining 40 rats were fed with high-fat diet to establish the model of obsesity IR; after 8 weeks, 24 rats with successful model of obsesity IR were randomly divided into a model group, an EA group and a sham EA group, 8 rats in each group. Eight weeks after model establishment, the rats in the EA group were intervened with EA at "Fenglong" (ST 40), "Zhongwan" (CV 12), "Guanyuan" (CV 4) and "Zusanli" (ST 36), with continuous wave, in frequency of 2 Hz, and current intensity of 1 mA, for 10 min each time. The rats in the sham EA group were intervened with EA at the points 5 mm next to the acupoints used in the EA group and no electricity was given; the sham EA was given for 10 min each time. Both the treatments were given once every other day for 8 weeks. The Lee's index and food intake were observed before the intervention as well as 2 weeks, 4 weeks, 6 weeks and 8 weeks into intervention; after the intervention, serum insulin (INS) and glucose infusion rate (GIR) were detected; serum cholecystokinin (CCK) level was detected by ELISA; c-fos expression in the area postrema (AP) and nucleus tractus solitarius (NTS) of medulla oblongata was detected by immunohistochemistry. RESULTS: Before the intervention as well as 2 weeks, 4 weeks, 6 weeks and 8 weeks into intervention, the Lee's index and food intake in the model group were higher than those in normal group (P<0.01). The Lee's index (6 weeks and 8 weeks into intervention) and food intake (4 weeks, 6 weeks and 8 weeks into intervention) in the EA group were lower than those in the model group and the sham EA group (P<0.05, P<0.01). After intervention, compared with the normal group, serum level of INS was increased (P<0.01), while GIR, serum CCK level, c-fos expression in AP and NTS in the model group were decreased (P<0.01, P<0.05). Compared with the model group and the sham EA group, serum level of INS in the EA group was decreased (P<0.01), and GIR, serum CCK level, c-fos expression in AP and NTS were increased (P<0.01, P<0.05). CONCLUSION: EA of "biaoben acupoint combination" could effectively reduce appetite, body fat and enhance insulin sensitivity in obese rats with IR. The mechanism may be related to the regulation of central sensitivity of CCK. 目的：观察“标本配穴”电针干预对胰岛素抵抗肥胖大鼠食欲、体脂含量、胰岛素敏感性以及脑肠肽胆囊收缩素（CCK）中枢敏感性的影响，探讨电针改善胰岛素抵抗肥胖的作用机制。方法：从50只8周龄健康SPF级Wistar雄性大鼠中随机挑选10只喂以普通饲料，8周后随机挑选其中8只作为正常组；另外40只喂以高脂饲料建立胰岛素抵抗肥胖模型，8周后，将24只造模成功的大鼠随机分为模型组、电针组和假电针组，每组 8只。造模8周后，电针组选取“丰隆”“中脘”“关元”“足三里”行电针干预，予连续波，频率2 Hz，电流强度 1 mA，每次10 min。假电针组于穴位向左旁开约 5 mm 浅刺，按电针组电极连接方式夹持电极，不予通电，每次10 min。两组均隔日1次，干预8周。分别在干预前，干预2、4、6、8周后观察各组大鼠Lee's指数、进食量；干预后，检测血清胰岛素（INS）含量、葡萄糖输注速率（GIR），ELISA法检测血清CCK水平，免疫组化法检测大鼠延髓最后区（AP）和孤束核（NTS）中c-fos表达。结果：干预前，干预2、4、6、8周后，模型组较正常组Lee's指数、进食量均升高（P<0.01）；电针组Lee's指数（干预6、8周）、进食量（干预4、6、8周）低于模型组和假电针组（P<0.05，P<0.01）。干预后，模型组大鼠血清INS水平较正常组升高（P<0.01），GIR、血清CCK水平、AP和NTS中c-fos表达均降低（P<0.01，P<0.05）；与模型组和假电针组干预后比较，电针组大鼠血清INS水平降低(P<0.01），GIR、血清CCK水平、AP和NTS中c-fos表达升高（P<0.01，P<0.05）。结论：“标本配穴”电针干预可以有效降低胰岛素抵抗肥胖大鼠的食欲及体脂含量，增强胰岛素敏感性，其机制可能与调控中枢对 CCK 的敏感性有关。.

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