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  • Title: Early myoblast differentiation in fast and slow types.
    Author: Sisto Daneo L, Cameron-Curry P, Corvetti G, Filogamo G.
    Journal: Basic Appl Histochem; 1987; 31(1):17-32. PubMed ID: 3297025.
    Abstract:
    Serial sections of stage 18-27 HH (3-5 days of incubation) chick embryo myotomes were investigated by electron microscope. Two morphologically different types of contractile elements (myoblasts and myotubes) with an exact and constant localization were identified. Myotome sections of other chick embryos in the same stages were examined with the immunofluorescence technique, after treatment with antisera directed against fast and slow adult myosins. As from stage 24 HH, some contractile elements react positively with anti-fast adult myosin antiserum, others with anti-slow antiserum. A very precise correspondence was constantly found between the ultrastructural and immunohistochemical findings. An identical pattern was observed in the buds of the fast-twitching pectoralis major and posterior latissimus dorsi and slow-twitching anterior latissimus dorsi muscles.
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