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  • Title: Overproduction and preliminary X-ray characterization of aspartate aminotransferase from Escherichia coli.
    Author: Kamitori S, Hirotsu K, Higuchi T, Kondo K, Inoue K, Kuramitsu S, Kagamiyama H, Higuchi Y, Yasuoka N, Kusunoki M.
    Journal: J Biochem; 1987 Mar; 101(3):813-6. PubMed ID: 3298223.
    Abstract:
    The aspartate aminotransferase of Escherichia coli was overproduced in cells after genetic manipulation, and was crystallized from a polyethylene glycol solution, pH 7.0. The crystals obtained were of good quality and had diffractions extending beyond 2.4 A. The space group and unit cell dimensions were determined with a precession camera and a four-circle diffractometer to be C222(1), and a = 157.1 A, b = 85.5 A, and c = 79.7 A, respectively. Only one protein subunit is contained in an asymmetric unit.
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