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  • Title: [On the role of N7 atoms of guanosine in tRNA-Phe (E. coli) in interaction with ribosomes].
    Author: Vlasov VV, Knorre DG, Skobel'tsyna LM.
    Journal: Biokhimiia; 1977 May; 42(5):784-7. PubMed ID: 329897.
    Abstract:
    The influence of alkylation of phe-tRNAPhe (E. coli) with 2',3'-O-[4-(N-2-chloroethyl-N-methylamino)-benzylidene]-uridine - 5' - methylphosphate on its ability to participate in non-enzymatic complex formation with ribosomes and poly-U was investigated. Phe-tRNAsPhe, containing alkylated guanosines at different positions, including anticodone, are active in binding with ribosomes. It is concluded, that N7 nitrogens of guanosine of the tRHAPhe are not elements, significant for the interaction with ribosomes.
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