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  • Title: Stability of Glucose Concentrations in Frozen Plasma.
    Author: Pleus S, Freckmann G, Baumstark A, Haug C.
    Journal: J Diabetes Sci Technol; 2022 Sep; 16(5):1096-1100. PubMed ID: 33034207.
    Abstract:
    BACKGROUND: Conflicting information is available regarding the stability of glucose concentrations in frozen plasma samples. Clinical trials could benefit from such long-term storage because it would allow usage of a central laboratory with higher-quality laboratory analyzers in contrast to mobile analyzers in a decentralized setting. METHODS: In this study, venous blood samples were collected in lithium-heparin gel tubes. Plasma was separated immediately after blood was drawn, and from each of the 21 plasma samples, 6 aliquots were prepared for measurement at 6 time points: immediately and after 2, 4, 6, 8, and 12 weeks. Between sampling and measurement, aliquots were stored at less than -20°C. Transport on dry ice was simulated by placing aliquots in a -80°C freezer for 5 days between weeks 8 and 12. Measurements were performed on a hexokinase-based laboratory analyzer.Average relative differences and corresponding 99% confidence intervals (CIs) were calculated between the stored aliquots' and the immediately measured aliquots' glucose concentrations. Glucose concentrations were deemed stable as long as average relative differences were ≤±2.5%. RESULTS: Over the whole 12-weeks duration, the largest average relative difference was -1.82% (99% CI: -2.25% to -1.39%). Shorter storage durations tended to lead to less bias. CONCLUSION: In this study, the stability of glucose concentrations in frozen plasma samples obtained with lithium-heparin gel tubes could be shown for up to 12 weeks. Future studies should be performed to assess whether this is independent of the glucose analyzer and the type of sampling tube used.
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