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  • Title: A simple differential immunoprecipitation assay of urinary acid and neutral alpha-glucosidases for glycogenosis II.
    Author: Tsuji A, Yang RC, Omura K, Imabayashi T, Suzuki Y.
    Journal: Clin Chim Acta; 1987 Aug 31; 167(3):313-20. PubMed ID: 3304722.
    Abstract:
    A specific assay for acid alpha-glucosidase in urine was developed to facilitate the diagnosis of glycogenosis II. This enzyme activity was calculated as a difference between the alpha-glucosidase activities before and after immunoprecipitation with antiserum to acid alpha-glucosidase. Acid alpha-glucosidase accounted for 86% of the total activity in control urine. All the cases of various clinical types of glycogenosis II showed either a marked decrease or a complete deficiency of this enzyme activity. A marked decrease of acid alpha-glucosidase was demonstrated by immunoblotting of the urine from patients with late-onset forms of this disease. These results indicate that assays of urinary acid alpha-glucosidase by this immunological method are useful for detection of the various types of glycogenosis II.
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