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  • Title: Enzyme-linked immunofiltration assay for rapid serodiagnosis of syphilis.
    Author: Ijsselmuiden OE, Meinardi MM, van der Sluis JJ, Menke HE, Stolz E, van Eijk RV.
    Journal: Eur J Clin Microbiol; 1987 Jun; 6(3):281-5. PubMed ID: 3305006.
    Abstract:
    A new rapid technique for detection of serum treponemal antibodies is described which is based on an enzyme-linked immunoassay using nitrocellulose as solid phase. With this technique antigen-antibody binding is accelerated by the filtration of the antibody solution through the antigen-coated nitrocellulose filter instead of its remaining over the solid phase for incubation. Test results are available in less than 15 min. Serum specimens from 255 syphilitics and 829 non-infected subjects were investigated. The sensitivity and specificity of the Treponema pallidum enzyme-linked immunofiltration assay were comparable to those of the Treponema pallidum haemagglutination assay and the fluorescent treponemal antibody absorption test.
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